Larry the Cable Guy Mater (voice) Owen Wilson Lightning McQueen (voice) Michael Caine Finn McMissile (voice) Emily Mortimer Holley Shiftwell (voice) John Turturro Francesco Bernoulli (voice) Eddie Izzard Sir Miles Axlerod (voice) Thomas Kretschmann Professor Z (voice) Guido Quaroni Guido (voice) Joe Mantegna Grem (voice) Peter Jacobson Acer (voice) Bruce Campbell Rod "Torque" Redline (voice) Tony Shalhoub Luigi (voice) Darrell Waltrip Darrell Cartrip (voice) Brent Musburger Brent Mustangburger (voice) Jason Isaacs Siddeley / Leland Turbo (voice) Stanley Townsend Victor Hugo / Vladimir Trunkov / Ivan the Tow Truck (voice) Lloyd Sherr Fillmore / Combat Ship (voice) Paul Dooley Sarge (voice) Sig Hansen Crabby (voice) Franco Nero Uncle Topolino (voice) Vanessa Redgrave The Queen / Mama Topolino (voice) Bonnie Hunt Sally Carrera (voice) Cheech Marin Ramone (voice) Jenifer Lewis Flo (voice) Michael Wallis Sheriff (voice) Katherine Helmond Lizzie (voice) John Ratzenberger Mack (voice) Jeff Garlin Otis (voice) Lewis Hamilton Lewis Hamilton (voice) Velibor Topic Alexander Hugo (voice) Brad Lewis Tubbs Pacer (voice) Richard Kind Van (voice) Edie McClurg Minny (voice) Teresa Gallagher Mater's Computer (voice) Jeff Gordon Jeff Gorvette (voice) John Lasseter Additional Voices (voice) Daisuke Tsutsumi Additional Voices (voice) Fernando Alonso Fernando Alonso (voice)